• Le 08 octobre 2019
    Campus centre ville

" Relations publiques et privées entre microbiote et anticorps "


Pr Guy GOROCHOV

Guy Gorochov first trained as a clinical hematologist in Paris, then as a post-doctoral fellow, first at the Weizmann Institute (Israel) working on the first generation of Chimeric Antigen Receptors (CARs) and then at the MRC laboratory of Molecular Biology in Cambridge (U.K.), working on antibody engineering. He is now co-director of the CIMI research centre (UPMC/Inserm U1135) in Paris. The team recently focused on the study of the balance between effector and regulatory T cells and on their relations with pathogens and commensals, very recently developed novel markers for the definition of human regulatory T cells and a platform dedicated to study the interface between adaptive immunity and gut microbiota.

1. Sialyl Lewis x (CD15s) identifies highly differentiated and most suppressive FOXP3high regulatory T cells in humans. M Miyara, D Chader, H Nishikawa, E Sage, D Bouvry, L.Claër, R Hingorani, R Balderas, J Rohrer, N Warner, A Chapelier, D Valeyre, L Musset, R Kannagi, S Sakaguchi, Z Amoura, G Gorochov. PNAS, 2015

2. Analysis of bacterial surface-specific antibodies in body fluids using bacterial flow cytometry, Fadlallah J, Moor K, Toska A, Sterlin D, Macpherson AJ, Gorochov G, Larsen M, Slack E. Nature Protocols, 2016.

3. Microbial ecology perturbation in human IgA deficiency. Fadlallah J, El Kafsi H, Sterlin D, Juste C, Parizot C, Dorgham K, Autaa G, Gouas D, Almeida M, Lepage P, Pons N, Le Chatelier E, Levenez F, Kennedy S, Galleron N, Pais de Barros JP, Malphettes M, Galicier L, Boutboul D, Mathian A, Miyara M, Oksenhendler E, Amoura Z, Doré J, Fieschi C, Dusko Ehrlich SSD, Larsen M, Gorochov G. Sci Transl Med. 2018.

4. Synergistic convergence of microbiota-specific systemic IgG and secretory IgA. Fadlallah J, Sterlin D, Fieschi C, Parizot C, Dorgham K, Autaa G, Ghillani-Dalbin P, Juste C, Lepage P, Malphettes M, Galicier L, Boutboul D, Clément K, André S, Marquet F, Tresallet C, Mathian A, Miyara M, Oksenhendler E, Amoura Z, Yssel H Larsen M, Gorochov G. Journal of Allergy and Clinical Immunology, 2018.
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Immune/bacterial interface in health and disease

Man has developed spectacular ways to protect himself from pathogens, while benefitting from unique and essential features of symbionts. We postulate that a perturbation of this balance is predisposing to, or is aggravating diseases such as systemic autoimmunity and cancer. The role played by IgA at the immune-microbiota interface was studied in controls and patients with selective IgA deficiency. Each individual targets a diverse, but nevertheless private microbiota repertoire. Partially overlapping anti-carbohydrate binding patterns, observed both at the isotypic and clonal level, could explain these apparently opposing features of IgA, being at the same time cross-reactive and selective in its interactions with the microbiota. Although absent from the gut, serum anti-microbiota IgG (mostly IgG2) are present in healthy individuals, and converge with secretory IgA to target the same bacterial cells. The latter observation emphasizes the close link between local and systemic anti-microbiota immunity.


Avec le soutien du consortium MiBioGate